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SRX11406228: WGS sequence for Chromobacterium haemolyticum strain HSC-31F16
1 ILLUMINA (Illumina HiSeq 2500) run: 459,009 spots, 204.9M bases, 127Mb downloads

Design: Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA was increased 2-fold, and PCR elongation time was increased to 45 s. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries were sequenced using an Illumina sequencer (HiSeq 2500) using a 250bp paired end protocol. Reads were adapter trimmed using Trimmomatic 0.30 with a sliding window quality cutoff of Q15. De novo assembly was performed on samples using SPAdes version 3.7.
Submitted by: Sierra College
Study: Characterization of violacein-producing bacteria and related species.
show Abstracthide Abstract
Isolates were obtained primarily by undergraduate students collecting environmental samples from a variety of habitats including fresh-water, soil and plant surfaces.
Sample:
SAMN13942215 • SRS9449949 • All experiments • All runs
Library:
Name: 28874_HSC31F16
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 459,009 spots, 204.9M bases, 127Mb
Run# of Spots# of BasesSizePublished
SRR15096377459,009204.9M127Mb2021-07-10

ID:
15260600

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